Graduate study
Subject Molecular biology 3. year general medicine, winter semester 2022, academic year 2023/2024
Subject description:
The graduate should acquire basic overview about molecular biology in medicine, knowledge concerning application of the methods of molecular biology in medicine general and in personalized medicine particularly, working with genomic internet brosers, understanding the basic principles of molecular diagnostics, precision medicine and interpretation of the results of the molecular-genetic examinations using databases.
Literature: Study material is uploaded in MS Teams
Recommended textbooks and databases:
T . Strachan, J. Goodship, P. Chinnery: Genetics and Genomics in Medicine, 2015 by Garland Science, Taylor and Francis Group, NewYork and London (selected chapters) ISBN 978-0-8153-4480-3
www.ncbi.nlm.nih.gov/omim/ - Online Medelian Inheritance in Man
Prerequisite: Good background knowledge of basic principles of molecular biology and classical genetics is expected
Lectures – Friday 2 PM - 3:45 PM EC MH4B
Classes - Department of Molecular Biology and Genomics, Mala hora 4C, 4th. fluor , room 5.51
1.week (Sept. 22rd) | Basics of molecular biology and the molecular nature of the human genome |
2.week (Sept. 29th) | Methods in molecular biology I - |
3. week (Oct. 6th) | Molecular biology of the genes, variant classification, and disease-causing pathogenic variants. |
4.week (Oct. 13th) | Molecular biology of cancer |
5. week (Oct. 20th) |
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6. week (Oct. 27th) | TEST (Lectures 1-4) 1:30 PM Groups 1,2,3,4,7 2PM Groups 5,6,7,8,9,10 Clinical applications of molecular biology II |
Practical classes 2.10-13.10. (week 3 and 4) |
Methods in molecular biology |
16.10.-27.10. (week 5 and 6) | Basics of molecular biology |
31.10.-11.11. (weeks 7 and 8) | Droplet digital PCR in tumor diagnostics, PCR in prenatal testing, an illustrative example of the project
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14.11-25.11. (weeks 9 and 10) 28.11-9.12. weeks 11 and 12 | Detection and interpretation of DNA variants, molecular diagnostics workflow and algorithm Presentation and discussion of projects, |
Attendance and participation:
Your attendance in classes is essential for 80%. If the student is ill, but will be able to learn, respectively. will be quarantined, remote connection to practical class will also be possible.
Grading: Your final grade from the course will be calculated as follows – 100% attendance classes (one absence can be excused for serious reasons) plus
Project | Test | Class 3-4 results each 5 % |
|
45 % | 45 % | 10 %
|
|
A/1 grade = 91-100 points C/2 grade =73-80 points E/3 grade =60-65 points
B/1,5 grade = 81-90 points D/2,5 grade =66-72 points FX grade=less than 60 points