Graduate study
Subject Molecular biology 3. year general medicine, winter semester 2024, academic year 2024/2025
Subject description:
The graduate should acquire basic overview about molecular biology in medicine, knowledge concerning application of the methods of molecular biology in medicine general and in personalized medicine particularly, working with genomic internet brosers, understanding the basic principles of molecular diagnostics, precision medicine and interpretation of the results of the molecular-genetic examinations using databases.
Literature: Study material is uploaded in MS Teams
Recommended textbooks and databases:
T . Strachan, J. Goodship, P. Chinnery: Genetics and Genomics in Medicine, 2015 by Garland Science, Taylor and Francis Group, NewYork and London (selected chapters) ISBN 978-0-8153-4480-3
www.ncbi.nlm.nih.gov/omim/ - Online Medelian Inheritance in Man
Prerequisite: Good background knowledge of basic principles of molecular biology and classical genetics is expected
Lectures – Friday 2 PM - 3:45 PM EC MH4B
Classes - Department of Molecular Biology and Genomics, Mala hora 4C, 4th. fluor , room 5.51
1. | Basics of molecular biology and the molecular nature of the human genome |
2. | Methods in molecular biology I - |
3. | Molecular biology of the genes, variant classification, and disease-causing pathogenic variants. |
4. | Molecular biology of cancer |
5. | Clinical applications of molecular biology I |
| 6. | TEST , Clinical applications in molecular biology II |
Practical classes |
|
1. | Fundamentals of molecular biology of the human genome and human genes |
| 2. | Examples of human genome variability - SNP |
| 3. | Method in molecular biology - ddPCR |
| 4. | First-generation sequencing and interpretation of variants |
5. | Fragment analysis. Example of genetic testing |
6. | Next-generation sequencing and interpretation of variants
|
| 7. | Molecular biology in forensics |
| 8. | Molecular biology in medicine - project preparations |
9. | Presentation and discussion of projects |
Attendance and participation:
Your attendance in classes is mandatory for 100%. In case of illness or other serious reason, it is possible to excuse non-participation in one class after personally delivering the confirmation. if you miss two or a maximum of three exercises, it is necessary to make up for the absence in the substitution week.
Grading: Your final grade from the subject will be calculated as follows – 100% attendance classes (one absence can be excused for serious reasons) plus
Test 50 % | Project 40 % | Activity (up-to-date information on options during the respective class) 10 %
|
A/1 grade = 91-100 points C/2 grade =73-80 points E/3 grade =60-65 points
B/1,5 grade = 81-90 points D/2,5 grade =66-72 points FX grade=less than 60 points