Department of experimental carcinogenesis
Head of department: Assoc. Prof. RNDr. Peter Kubatka, PhD.
Department of Experimental Carcinogenesis, Division of Oncology is focused on the chemoprevention of experimental breast carcinoma using animal models. Using the synthetic substances, chemopreventive effects of retinoids, non-steroidal anti-inflammatory drugs, selective estrogen receptor modulators, aromatase inhibitors, antidiabetic agents, statins, and melatonin have been evaluated in recent years. More recently, the department is focused on assessing the chemopreventive potential of plant natural substances in experimental mammary carcinogenesis.
Methods of animal modeling are based on analyses of the basic parameters of experimental carcinogenesis in laboratory rats (frequency, incidence, latency, and tumor volume) and histological typing and grading of mammary tumors. In the syngeneic (4T1) mouse model, we analyze the therapeutic effect of natural substances and survival of the animals. In cooperation with the Department of Histology and Embryology JMF CU, we perform immunohistochemical analyzes of the mechanism of action (epigenetics - chemical modifications of histones, apoptosis, angiogenesis, proliferation, oxidative damage of proteins and lipids, and parameters of cancer stem cells). In collaboration with the Department of Molecular Oncology and Diagnostics of the Division of Oncology and the Department of Molecular Biology, JFM CU, we perform further epigenetic evaluations - pyrosequencing analyzes of the degree of the promoters methylations and expressions of selected miRNA. In cooperation with the Department of Pharmacology of FM UPJŠ Košice, we carry out in vitro studies using human mammary adenocarcinoma cells (MCF-7 or MDA) where we perform a cytotoxicity test (MMT), analyses of apoptosis (annexinV, cell cycle analysis, changes in mitochondrial membrane potential, and ROS analysis), proliferation (BrdU analysis), and angiogenesis (HUVEC lines).