Jessenius Faculty of Medicine
in Martin
Comenius University in Bratislava

Department of experimental biochemistry

 

Head of department: Prof. RNDr. Peter Kaplán, PhD.

Department is focused on study of molecular mechanisms of heart and brain ageing as well as mechanisms of ischemia-reperfusion injury of heart and brain, study of molecular mechanisms of ischemia-induced neuronal cell death, identification of target proteins playing a role in protection of neuronal cells from ischemia-reperfusion injury.

Department is using imaging mass spectrometer IMScope TRIO for identification of metabolites and study of metabolite modifications in tissues as a consequence of pathologic processes of CNS, pharmacokinetic analysis of therapeutic drugs, localization of native drugs and their metabolites in tissues. In addition, mass spectrometer Iontrap-TOF is used for identification of proteins and their posttranslation modifications as a consequence of aging and ischemia.

Fig. 1. IMScope TRIO (A), imaging of a part of mouse brain by light microscope (B) and MS system m/z 772,49 (C), 848,61 (D) and 924,60 (E)